Holding micropipettes are indispensable tools in embryology labs, playing a critical role in procedures like ICSI (Intracytoplasmic Sperm Injection) and embryo biopsy. However, even experienced embryologists can make mistakes when using these precision instruments, leading to compromised results or damage to delicate embryos.

In this article, we’ll explore five common mistakes when using holding micropipettes and provide actionable tips to avoid them. Whether you’re a seasoned embryologist or new to the field, these insights will help you optimize your technique and achieve better outcomes.

1. Using the Wrong Size or Type of Micropipette

The Mistake:

Not all holding micropipettes are created equal. Using the wrong size or type (e.g., too large or too small) can lead to poor embryo handling, zona pellucida damage, or inefficient sperm immobilization.

How to Avoid It ?

• Match the Micropipette to the Procedure:

  • For ICSI, use a holding pipette with an inner diameter slightly larger than the oocyte (e.g., 30-40 µm).

  • For embryo biopsy, choose a pipette with a finer tip to minimize trauma.

• Consult Product Guidelines: Always refer to the manufacturer’s recommendations for specific applications.

• Test Before Use: Perform trial runs with non-viable oocytes or embryos to ensure the pipette is a good fit.

2. Improper Positioning of the Micropipette

The Mistake:

Holding micropipettes must be positioned at the correct angle and depth to stabilize the embryo or oocyte effectively. Incorrect positioning can cause slippage, zona pellucida damage, or incomplete immobilization.

How to Avoid It ?

• Maintain a 30-45 Degree Angle: This angle ensures optimal contact with the embryo or oocyte without excessive pressure.

• Avoid Over-Insertion: Insert the pipette just enough to hold the embryo securely. Over-insertion can damage the zona pellucida or cytoplasm.

• Use a Stable Platform: Ensure the microscope stage and micromanipulators are properly calibrated to avoid sudden movements.

3. Applying Too Much or Too Little Suction

The Mistake:

Inconsistent suction pressure is a common issue. Too much suction can deform or damage the embryo, while too little suction may fail to hold it securely.

How to Avoid It ?

• Calibrate Suction Pressure: Use a microinjector with precise pressure control and calibrate it regularly.

• Practice Gentle Handling: Apply just enough suction to hold the embryo or oocyte without causing visible deformation.

• Monitor Visual Feedback: Watch for signs of stress, such as zona pellucida stretching or cytoplasmic leakage.

4. Neglecting Proper Cleaning and Sterilization

The Mistake:

Holding micropipettes are reusable tools, but improper cleaning and sterilization can lead to contamination, affecting embryo viability and lab results.

How to Avoid It ?

• Follow a Strict Cleaning Protocol:

  • Rinse with distilled water immediately after use.

  • Soak in a enzymatic cleaner to remove organic debris.

  • Sterilize using autoclaving or chemical sterilants approved for medical devices.

• Inspect for Damage: Check for cracks or clogs before reuse. Damaged pipettes should be discarded.

• Store Properly: Keep cleaned pipettes in a sterile, dust-free environment.

5. Failing to Replace Worn-Out Micropipettes

The Mistake:

Over time, holding micropipettes can wear out, leading to reduced performance, increased risk of contamination, or even breakage during use.

How to Avoid It ?

• Monitor Usage: Keep track of how many cycles each pipette has been used for.

• Look for Signs of Wear: Replace pipettes with visible damage, such as scratches, bends, or dull tips.

• Invest in High-Quality Pipettes: Choose durable, medical-grade micropipettes from trusted manufacturers like Monash Biotech.

Holding micropipettes are essential tools in embryology, but their effectiveness depends on proper use and maintenance. By avoiding these five common mistakes, you can improve your technique, protect delicate embryos, and achieve better outcomes in your IVF lab.

At Monash Biotech, we design our holding micropipettes with precision and durability in mind, ensuring they meet the highest standards for embryology procedures. Whether you’re performing ICSI, embryo biopsy, or other ART procedures, our tools are built to deliver consistent, reliable results.

Pro Tip: For more insights on optimizing your IVF lab techniques, explore our blog or contact our team for personalized recommendations.

Looking for high-quality holding micropipettes? Visit Monash Biotech’s Product Page to explore our range of embryology tools designed for precision and reliability.