Can cryopreserved sperm affect micropipette performance ?
Debris & Aggregates: Thawing can release membrane fragments that clog narrow bores—perform thorough wash steps.
Osmotic Shifts: Rapid volume changes may introduce microbubbles; ensure gentle resuspension.
Viscosity Changes: Post-thaw medium may be more viscous—adjust aspiration pressure accordingly.
Holding Micropipette
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