How to immobilize sperm properly before aspiration ?
Tail Snip: Gently press the pipette tip against the tail until movement ceases.
PVP Droplet: Draw sperm into a small PVP (polyvinylpyrrolidone) drop—its viscosity further halts motility.
Confirm Stationary Tail: Always verify under high magnification before moving the sperm to the oocyte.
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